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What is Cryo-Fluorescence Tomography?

By: Natalie Paskoski, NSH Communications Specialist


Cryo-fluorescence tomography is an imaging modality that aims to bridge the gap between in vivo and ex vivo imaging. So, what does that mean? If you are familiar with Latin, you may be able to figure out that in vivo means “within the living”, while ex vivo means “out of the living”. Think of in vivo imaging as modalities such as MRI’s or PET scans, where the imaging is done on a large, living, specimen, like a person going through the MRI machine. These methods are important because it lets you get a better view of the problem as a whole, instead of just a small section, and allows for monitoring of a tumor over time, but in doing that there are issues with low resolution and lack of sensitivity and specificity. On the other hand, microscopy provides high resolution but of a small volume sample, outside of the living organism.


What is CFT and how does it bridge this gap?

Cryo-fluorescence tomography is an imaging modality that takes ex vivo fluorescence from 2D sections and combines it into a 3D image. So they prepare the sample, which may include adding tracers. They may also do other in vivo imaging, for a multi-modality approach. The sample is then frozen in a block, the block is sectioned, and the block face is imaged with white light and fluorescence imaging. You might also collect sections here to do other testing, such as IHC later on. The images are then aligned and rendered in 3D using a software tool. The idea here is that you end up with both molecular information and structural information that you can overlay to get a better sense of the total picture that is lacking from just an MRI on its own or just a tissue sample on its own.


Want to learn more about the science behind cryo-fluorescence tomography? Join us May 26th at 1PM ET for our May Laboratory Webinar, Cryo-Fluorescence Tomography: A Technique to Bridge Multi-Resolution Multi-Modal Assays, presented by Mohammed Farhoud, MS, Emit Imaging. Your entire lab can view the webinar and earn a CEU credit for one low price.


https://www.novoprolabs.com/support/articles/what-is-the-difference-between-ex-vivo-in-vivo-and-in-vitro-201802261300.html

https://www.hindawi.com/journals/bmri/2014/819324/

https://cdn2.hubspot.net/hubfs/4366084/CFT_July%202019.pdf

https://lp.invicro.com/utilization-of-3d-fluorescent

https://cancerres.aacrjournals.org/content/78/13_Supplement/LB-366

https://www.youtube.com/watch?v=56dXbz4Zfyg






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